Preparation of Ultra-Violet Fluorescent Photographs of Steroids

Physics

Scientific paper

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Scientific paper

A NUMBER of methods have been used to record photographically the fluorescence of various substances present on paper chromatograms when excited by ultra-violet light. Markham and Smith1-3 used a system consisting of a quartz mercury vapour lamp and a set of two filters containing chlorine gas and a solution of NiSO4.7H2O and CoSO4.7H2O, respectively. Bernasconi, et al.4 used a mercury vapour lamp, a red-purple filter, a cobalt sulphate and nickel sulphate filter, and a 3 mm. thick piece of `Plexiglass'. Abelson5 used an ultra-violet hand lamp with a Kodak 2A gelatin filter enclosed in glass plates.

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