Predicting Knot or Catenane Type of Site-Specific Recombination Products

Details Predicting Knot or Catenane Type of Site-Specific Recombination Products Predicting Knot or Catenane Type of Site-Specific Recombination Products

2007-07-25

arxiv.org/abs/0707.3775v2

Biology

Quantitative Biology

Biomolecules

17 pages, 4 figures. Revised to include link to the companion paper, arXiv:0707.3896v1, that provides topological proofs under

Scientific paper

Site-specific recombination on supercoiled circular DNA yields a variety of knotted or catenated products. We develop a model of this process, and give extensive experimental evidence that the assumptions of our model are reasonable. We then characterize all possible knot or catenane products that arise from the most common substrates. We apply our model to tightly prescribe the knot or catenane type of previously uncharacterized data.

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