Physics – Biological Physics
Scientific paper
2011-04-12
Physics
Biological Physics
12 pages, 7 figures
Scientific paper
DNA interactions with polycations are not only important for our understanding of chromatin compaction but also for characterizing DNA-binding proteins involved in transcription, replication and repair. DNA is known to form several types of liquid-crystalline phases depending, among other factors, on polycation structure and charge density. Theoretical studies and simulations have predicted the wrapping of DNA around spherical positively charged polycations. As a potential mimic of the histone octamer or other DNA wrapping proteins, poly(amido amine) generation 6 (PAMAM6) dendrimers have been chosen for our study. The self-assembly of DNA induced by PAMAM6 has been investigated using small angle X-ray scattering (SAXS) in order to reveal the assemblies' structure dependence on the pH of the environment and on dendrimers concentration. We demonstrate that at pH 8.5 dense phases are formed and characterized by a 2D-columnar hexagonal lattice which is transformed into a 3D hexagonal lattice with increasing dendrimer concentration (charge ratio N/P). Moreover, a systematic analysis of the scattering data collected from diluted samples at pH 8.5 and 5.5 have led us to propose a wrapping scenario of DNA around PAMAM6 at pH 5.5.
Dootz Rolf
Pfohl Thomas
Toma Adriana Cristina
No associations
LandOfFree
PAMAM6 dendrimers and DNA: pH dependent "beads-on-a-string" behavior revealed by small angle X-ray scattering does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with PAMAM6 dendrimers and DNA: pH dependent "beads-on-a-string" behavior revealed by small angle X-ray scattering, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and PAMAM6 dendrimers and DNA: pH dependent "beads-on-a-string" behavior revealed by small angle X-ray scattering will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-731059