Optothermal Molecule Trap

Details Optothermal Molecule Trap Optothermal Molecule Trap

2006-06-14

arxiv.org/abs/physics/0606121v1

Physics

Biological Physics

9 pages, 6 figures

Scientific paper

Thermophoresis moves molecules along temperature gradients, typically from hot to cold. We superpose fluid flow with thermophoretic molecule flow under well defined microfluidic conditions, imaged by fluorescence microscopy. DNA is trapped and accumulated 16-fold in regions where both flows move in opposite directions. Strong 800-fold accumulation is expected, however with slow trapping kinetics. The experiment is equally described by a three-dimensional and one-dimensional analytical model. As an application, we show how a radially converging temperature field confines short DNA into a 10 um small spot.

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