Observation of Multiphoton-induced Fluorescence from Nano Graphene Oxide and Its Applications in In vitro and In vivo Bioimaging

Details Observation of Multiphoton-induced Fluorescence from Nano Graphene Oxide and Its Applications in In vitro and In vivo Bioimaging Observation of Multiphoton-induced Fluorescence from Nano Graphene Oxide and Its Applications in In vitro and In vivo Bioimaging

2012-02-09

arxiv.org/abs/1202.1975v1

Physics

Optics

submitted to Angew. Chem. Int. Ed. on Jan 25, 2012

Scientific paper

In the present paper, we observed both two-photon and three-photon induced distinct photoluminescence from GO nanoparticles under fs laser excitation. Conjugated with PEG molecules, GO nanoparticles exhibited high chemical stability, and could effectively label HeLa cells. Imaged with a two-photon scanning microscope, GO nanoparticles were observed to localize in the mitochondria, endoplasmic reticulum, Golgi and lysosome of HeLa cells. Furthermore, GO nanoparticles were micro-injected into the brain of a black mouse, and in vivo two-photon luminescence imaging illustrated that GO nanoparticles located at 300 {\mu}m depth in the brain could be clearly distinguished.

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