Non-random coil behavior as a consequence of extensive PPII structure in the denatured state

Details Non-random coil behavior as a consequence of extensive PPII structure in the denatured state Non-random coil behavior as a consequence of extensive PPII structure in the denatured state

2008-07-30

arxiv.org/abs/0807.4765v1

J. Mol. Biol. 382, 203 (2008)

Biology

Quantitative Biology

Biomolecules

32 pages, 4 figures, 1 table

Scientific paper

Unfolded proteins may contain native or non-native residual structure, which has important implications for the thermodynamics and kinetics of folding as well as for misfolding and aggregation diseases. However, it has been universally accepted that residual structure should not affect the global size scaling of the denatured chain, which obeys the statistics of random coil polymers. Here we use a single-molecule optical technique, fluorescence correlation spectroscopy, to probe the denatured state of set of repeat proteins containing an increasing number of identical domains, from two to twenty. The availability of this set allows us to obtain the scaling law for the unfolded state of these proteins, which turns out to be unusually compact, strongly deviating from random-coil statistics. The origin of this unexpected behavior is traced to the presence of extensive non-native polyproline II helical structure, which we localize to specific segments of the polypeptide chain. We show that the experimentally observed effects of PPII on the size scaling of the denatured state can be well-described by simple polymer models. Our findings suggest an hitherto unforeseen potential of non-native structure to induce significant compaction of denatured proteins, affecting significantly folding pathways and kinetics.

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