Molecular fluorescence measurements with a charge-coupled device detector

Physics

Scientific paper

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Charge Coupled Devices, Fluorescence, Molecular Excitation, Molecular Spectroscopy, Raman Spectra, Spectrometers, Anthracene, Emission Spectra, Ethyl Alcohol, Mercury Lamps, Optical Pumping, Spectral Sensitivity

Scientific paper

A novel fluorescence spectrometer is described using a two-dimensional charge-coupled device as the detector and a mercury pen lamp as the excitation source. The fluorescence spectra and intensity of anthracene in ethanol standards at concentrations ranging from 0.0001 to 10 to the minus 11th power M are shown. The limit of detection measured is 10 to the minus 12th power M, or 227 parts-per-quadrillion. The linear dynamic range obtained with this instrument is 6 orders of magnitude with excellent linearity using 10 second signal integration time.

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