Molecular analysis of mutagenesis by high let radiation

Details Molecular analysis of mutagenesis by high let radiation Molecular analysis of mutagenesis by high let radiation

Oct 1994

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1994adspr..14..355h&link_type=abstract

Advances in Space Research, Volume 14, Issue 10, p. 355-361.

Physics

3

Scientific paper

Mutation induction by high linear energy transfer [LET] alpha particles and gamma-rays was scored in the human hamster hybrid [AL] cells. Southern blotting technique was used to analyse the molecular changes in the DNA from both the HGPRT- and S1- mutants. Dose dependent mutagenesis in the AL cells irradiated with the charged particles was higher by almost 20 fold at the S1 than the corresponding HGPRT locus. Southern analysis of the mutants induced by the high LET particles showed mostly multilocus deletion at both the HGPRT and S1 genes.

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