Micro-Bioreactor Mimicking a Cellular Environment

Details Micro-Bioreactor Mimicking a Cellular Environment Micro-Bioreactor Mimicking a Cellular Environment

2006-05-17

arxiv.org/abs/physics/0605145v1

Physics

Biological Physics

Scientific paper

We report that a cell-sized water droplet (CWD) with a diameter of several tens of microns can serve as a micro-biochemical reactor. Since the droplet inner surface is covered by a phospholipid layer, it provides an environment similar to that in living cells. The CWD is manipulated with laser tweezers and we monitor the time-development of biochemical reactions in a single CWD by fusing two droplets which contain a substrate and an enzyme, respectively. As actual examples of enzymatic reactions, we present results on the reaction of calcein with esterase and on the expression reaction of green fluorescent protein.

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