Methane-producing bacteria: natural fractionations of the stable carbon isotopes

Physics

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Scientific paper

The 13 C / 12 C fractionation factors ( CO 2 / CH 4 ) for the reduction of CO 2 to CH 4 by pure cultures of methane-producing bacteria are, for Methanosarcina barkeri at 40°C, 1.045 ± 0.002; for Methanobacterium strain M.o.H. at 40°C, 1.061 ± 0.002; and, for Methanobacterium thermoautotrophicum at 65°C, 1.025 ± 0.002. These observations suggest that the acetic acid used by acetate dissimilating bacteria, if they play an important role in natural methane production, must have an intramolecular isotopic fractionation ( CO 2 H / CH 3 ) approximating the observed CO 2 / CH 4 fractionation.

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