Measuring Total and Germinable Spore Populations

Details Measuring Total and Germinable Spore Populations Measuring Total and Germinable Spore Populations

Oct 2011

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2011epsc.conf.1249n&link_type=abstract

EPSC-DPS Joint Meeting 2011, held 2-7 October 2011 in Nantes, France. http://meetings.copernicus.org/epsc-dps2011, p.1249

Computer Science

Scientific paper

It has been shown that bacterial endospores can be enumerated using a microscopy based assay that images the luminescent halos from terbium ions bound to dipicolinic acid, a spore specific chemical marker released upon spore germination. Further development of the instrument has simplified it towards automation while at the same time improving image quality. Enumeration of total spore populations has also been developed allowing measurement of the percentage of viable spores in any population by comparing the germinable/culturable spores to the total. Percentage viability will allow a more quantitative comparison of the ability of spores to survive across a wide range of extreme environments.

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