Physics – Chemical Physics
Scientific paper
2004-03-20
Physics
Chemical Physics
1 figure
Scientific paper
This paper presents a diffusion experimental study for ternary lysozyme-Na$_2$SO$_4$-water system, from moderate precipitant concentrations into the supersaturated region and provides a complete set of four diffusion coefficients. These data are important in order to provide accurate models of protein diffusion with applications in growth of protein crystals for X-ray diffraction studies. All three-component mutual-diffusion experiments reported here were performed by Rayleigh interferometry at pH$=4.5$, T$=25^o$ C and at a mean lysozyme concentration (average of top and bottom solution concentrations) of 0.6 mM (8.6 mg/mL). Four experiments, with different combinations of protein and Na$_2$SO$_4$ concentration differences, were performed at each of five mean Na$_2$SO$_4$ concentrations (0.1, 0.25, 0.5, 0.65 and 0.8 M), for a total of 20 experiments. In addition, we have measured dynamic light-scattering diffusion coefficients of the ternary system lysozyme chloride-Na$_2$SO$_4$-water.
Albright John G.
Buzatu Daniela
Buzatu Florin D.
Petrescu E.
No associations
LandOfFree
Measurements of Multicomponent Diffusion Coefficients for Lysozyme Chloride in Water and Aqueous Na$_2$SO$_4$ does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Measurements of Multicomponent Diffusion Coefficients for Lysozyme Chloride in Water and Aqueous Na$_2$SO$_4$, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Measurements of Multicomponent Diffusion Coefficients for Lysozyme Chloride in Water and Aqueous Na$_2$SO$_4$ will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-701434