Irradiation influence on the detection of genetic-modified soybeans

Details Irradiation influence on the detection of genetic-modified soybeans Irradiation influence on the detection of genetic-modified soybeans

Sep 2004

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2004rapc...71..491v&link_type=abstract

Radiation Physics and Chemistry, Volume 71, Issue 1-2, p. 491-494.

Physics

Gamma Radiation, Food Irradiation Detection, Soybean, Pcr

Scientific paper

Three soybean varieties were analyzed to evaluate the irradiation influence on the detection of genetic modification. Samples were treated in a 60Co facility at dose levels of 0, 500, 800, and 1000Gy. The seeds were at first analyzed by Comet Assay as a rapid screening irradiation detection method. Secondly, germination test was performed to detect the viability of irradiated soybeans. Finally, because of its high sensitivity, its specificity and rapidity the polimerase chain reaction was the method applied for genetic modified organism detection. The analysis of DNA by the single technique of microgel electrophoresis of single cells (DNA Comet Assay) showed that DNA damage increased with increasing radiation doses. No negative influence of irradiation on the genetic modification detection was found.

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