Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope

Details Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope

2009-08-10

arxiv.org/abs/0908.1177v2

Physics

Optics

11 pages, 5 figures, supplemental info: http://minty.stanford.edu/papers/Publications/McHale10aSI.pdf

Scientific paper

We derive the statistics of the signals generated by shape fluctuations of large molecules studied by feedback tracking microscopy. We account for the influence of intramolecular dynamics on the response of the tracking system, and derive a general expression for the fluorescence autocorrelation function that applies when those dynamics are linear. We show that tracking provides enhanced sensitivity to translational diffusion, molecular size, heterogeneity and long time-scale decays in comparison to traditional fluorescence correlation spectroscopy. We demonstrate our approach by using a three-dimensional tracking microscope to study genomic $\lambda$-phage DNA molecules with various fluorescence label configurations.

Affiliated with

Also associated with

No associations

Rating

Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Intramolecular fluorescence correlation spectroscopy in a feedback tracking microscope will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-120062