Internal strain regulates the nucleotide binding site of the kinesin leading head

Details Internal strain regulates the nucleotide binding site of the kinesin leading head Internal strain regulates the nucleotide binding site of the kinesin leading head

2007-02-07

arxiv.org/abs/q-bio/0702012v1

PNAS (2007) vol 104, 2175-2180

Biology

Quantitative Biology

Biomolecules

34 pages, 9 Figures

Scientific paper

10.1073/pnas.0610939104

In the presence of ATP, kinesin proceeds along the protofilament of microtubule by alternated binding of two motor domains on the tubulin binding sites. Since the processivity of kinesin is much higher than other motor proteins, it has been speculated that there exists a mechanism for allosteric regulation between the two monomers. Recent experiments suggest that ATP binding to the leading head domain in kinesin is regulated by the rearward strain built on the neck-linker. We test this hypothesis by explicitly modeling a $C_{\alpha}$-based kinesin structure whose both motor domains are bound on the tubulin binding sites. The equilibrium structures of kinesin on the microtubule show disordered and ordered neck-linker configurations for the leading and the trailing head, respectively. The comparison of the structures between the two heads shows that several native contacts present at the nucleotide binding site in the leading head are less intact than those in the binding site of the rear head. The network of native contacts obtained from this comparison provides the internal tension propagation pathway, which leads to the disruption of the nucleotide binding site in the leading head. Also, using an argument based on polymer theory, we estimate the internal tension built on the neck-linker to be f~(12-15) pN. Both of these conclusions support the experimental hypothesis.

Affiliated with

Also associated with

No associations

Rating

Internal strain regulates the nucleotide binding site of the kinesin leading head does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Internal strain regulates the nucleotide binding site of the kinesin leading head, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Internal strain regulates the nucleotide binding site of the kinesin leading head will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-219274