Interacion of Heavy Metal Ions with C-Phycocyanin: Binding Isotherms and Cooperative Effects

Details Interacion of Heavy Metal Ions with C-Phycocyanin: Binding Isotherms and Cooperative Effects Interacion of Heavy Metal Ions with C-Phycocyanin: Binding Isotherms and Cooperative Effects

2007-07-20

arxiv.org/abs/0707.3019v1

Physics

Medical Physics

10 pages, 0 figures

Scientific paper

The binding constant of copper(II) ions to C-PC were determined at different ionic strengths from binding isotherms by equilibrium dialysis and flame atomic absorption spectroscopy. Fluorescence and absorbtion spectroscopy provides insight of metal-C-phycocyanin interactions. Fluorescence measurements demonstrate C-PC quenching of heavy metal ions emission intensities. Stern-Volmer quenching constants were obtained from the linear quenching plots. Blue shifts in the fluorescence spectra were observed during metal binding to C-PC. It was shown, that between bound metal ions in C-PC there exists positive cooperativity.

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