Instrumentation aspects for spaceborn critical point and aggregation studies on the basis of various test data gathered by ground laboratory equipment

Details Instrumentation aspects for spaceborn critical point and aggregation studies on the basis of various test data gathered by ground laboratory equipment Instrumentation aspects for spaceborn critical point and aggregation studies on the basis of various test data gathered by ground laboratory equipment

Sep 1994

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1994spie.2210..223d&link_type=abstract

Proc. SPIE Vol. 2210, p. 223-236, Space Optics 1994: Space Instrumentation and Spacecraft Optics, Thierry M. Dewandre; Joachim J

Physics

Optics

Scientific paper

Time averaged static light scattering (SLS) intensity collections on suspensions with defined particle dimensions and concentrations were achieved by utilization of the Zeiss SG 100 goniometer scatterometer. The bidirectional straylight distribution functions (BSDF) of suspensions containing 36 mg/ml lysozyme with NaCl-buffer and latex standards with diameters of 90 nm, 270 nm, 310 nm, 500 nm and 3100 nm in various concentrations are given. The measurements clearly reveal absence of complex Mie light intensity distributions to which presence is often referred to. Presented data collection achieved with commercial equipment would enable extraction of instrument designs appropriate for operation of dynamic light scattering (DLS) in reduced gravity environments. Optoelectronics and fiber optics of the multiangle Coulter N4MD submicron analyzer are concisely described and analyzed. DLS is introduced concerning baseline determination and applied evaluation procedures. Consequences of analysis yielded potential advancements to two optomechanics (optrode; lens protein analyzer LPA and goniometer device; DIMINIGON-A). Their operation is demonstrated in linkage with a 288 real time correlator (ALV-Langen). Experimental results achieved during aggregation and crystallization of lysozyme together with detection of intercalation between DNA and doxorubicin (antitumor therapeutics) are included. Results of viscosity measurements under low shear rates as function of the protein concentrations will be presented.

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