Physics – Biological Physics
Scientific paper
2009-10-15
JAP, 105, 102036 (2009)
Physics
Biological Physics
mini-overview, a minor error in the explanatory material has been corrected
Scientific paper
10.1063/1.3116143
We discuss the Surface-Plasmon-Resonance (SPR) technique based on Fourier -Transform - InfraRed (FTIR) spectrometry. We explore the potential of the infrared surface plasmon resonance technique for biological studies in aqueous solutions and compare it to the conventional surface plasmon technique operating in the visible range. We demonstrate that the sensitivity of the SPR technique in the infrared range is not lower and in fact is even higher. We show several examples of applying FTIR-SPR for biological studies: (i) monitoring D-glucose concentration in solution, and (ii) measuring D-glucose uptake by erythrocytes in suspension. We emphasize the advantages of infrared SPR for studying living cell cultures and show how this technique can be used for characterization of (i) cholesterol penetration into plasma membrane, and (ii) transferrin-induced clathrin-mediated endocytosis.
Aroeti B.
Davidov D.
Golosovsky Michael
Lirtsman V.
Yashunsky V.
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