In situ laser power measurement at the focus of microscope objectives used in optical tweezers

Details In situ laser power measurement at the focus of microscope objectives used in optical tweezers In situ laser power measurement at the focus of microscope objectives used in optical tweezers

Mar 2005

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2005amjph..73..201v&link_type=abstract

American Journal of Physics, Volume 73, Issue 3, pp. 201-205 (2005).

Physics

1

Laboratory Experiments And Apparatus, Mechanical Effects Of Light On Atoms, Molecules, Electrons, And Ions, Wave Propagation, Transmission And Absorption, Permittivity

Scientific paper

We discuss measurements of the laser power at the focus of high numerical aperture objectives used in optical microscopy and optical tweezers. For a given power, the focused incident laser beam heats a small mercury bead that jumps when it reaches the boiling temperature of water, the medium used in the experiments. From the size of the mercury beads, the heat conductivities of water and glass-slide, and the absorption coefficient of mercury for the laser wavelength used, the incident power can be obtained using the solution of the associated heat problem. This problem is mapped onto the problem of a charged conducting sphere embedded in a semi-infinite medium of dielectric constant ɛ1, separated from another semi-infinite medium of dielectric constant ɛ2.

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