Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy

Details Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy

2009-11-26

arxiv.org/abs/0911.5040v1

Physics

Optics

Scientific paper

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments.

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