Identification of irradiated wheat by germination test, DNA comet assay and electron spin resonance

Details Identification of irradiated wheat by germination test, DNA comet assay and electron spin resonance Identification of irradiated wheat by germination test, DNA comet assay and electron spin resonance

Mar 2002

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2002rapc...63..423b&link_type=abstract

Radiation Physics and Chemistry, Volume 63, Issue 3-6, p. 423-426.

Physics

1

Scientific paper

In several countries, there has been an increase in the use of radiation for food processing thus improving the quality and sanitary conditions, inhibiting pathogenic microorganisms, delaying the natural aging process and so extending product lifetime. The need to develop analytical methods to detect these irradiated products is also increasing. The goal of this research was to identify wheat irradiated using different radiation doses. Seeds were irradiated with a gamma 60Co source (Gammacell 220 GC) in the Centro de Energia Nuclear na Agricultura and the Instituto de Pesquisas Energéticas e Nucleares. Dose rate used were 1.6 and 5.8kGy/h. Applied doses were 0.0, 0.10, 0.25, 0.50, 0.75, 1.0, and 2.0kGy. After irradiation, seeds were analysed over a 6 month period. Three different detection methods were employed to determine how irradiation had modified the samples. Screening methods consisted of a germination test measuring the inhibition of shooting and rooting and analysis of DNA fragmentation. The method of electron spin resonance spectroscopy allowed a better dosimetric evaluation. These techniques make the identification of irradiated wheat with different doses possible.

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