Identification of irradiated refrigerated pork with the DNA comet assay

Details Identification of irradiated refrigerated pork with the DNA comet assay Identification of irradiated refrigerated pork with the DNA comet assay

Sep 2004

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2004rapc...71..185a&link_type=abstract

Radiation Physics and Chemistry, Volume 71, Issue 1-2, p. 185-187.

Physics

1

Gamma Radiation, Food Irradiation Detection, Dna Comet Assay, Pork Meat

Scientific paper

Food irradiation can contribute to a safer and more plentiful food supply by inactivating pathogens, eradicating pests and by extending shelf-life. Particularly in the case of pork meat, this process could be a useful way to inactivate harmful parasites such as Trichinella and Taenia solium. Ionizing radiation causes damage to the DNA of the cells (e.g. strand breaks), which can be used to detect irradiated food. Microelectrophoresis of single cells (``Comet Assay'') is a simple and rapid test for DNA damage and can be used over a wide dose range and for a variety of products. Refrigerated pork meat was irradiated with a 60Co source, Gammacell 220 (A.E.C.L.) installed in IPEN (Sa~o Paulo, Brazil). The doses given were 0, 1.5, 3.0 and 4.5kGy for refrigerated samples. Immediately after irradiation the samples were returned to the refrigerator (6°C). Samples were kept in the refrigerator after irradiation. Pork meat was analyzed 1, 8 and 10 days after irradiation using the DNA ``Comet Assay''. This method showed to be an inexpensive and rapid technique for qualitative detection of irradiation treatment.

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