Physics
Scientific paper
Sep 2004
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2004rapc...71..193m&link_type=abstract
Radiation Physics and Chemistry, Volume 71, Issue 1-2, p. 193-196.
Physics
1
Gamma Radiation, Detection, Dna Comet Assay, Half-Embryo Test, Papaya, Melon, Watermelon
Scientific paper
Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.
Delincée Henry
Mancini-Filho Jorge
Marin-Huachaca Nélida Simona
Villavicencio Anna Lúcia C. H.
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