Physics – Biological Physics
Scientific paper
2001-08-14
Nuclear Instruments & Methods in Physics Research A 448 (2000) 225-260
Physics
Biological Physics
PDF: 9 pages, 3 figures; sourse in MS Word
Scientific paper
10.1016/S0168-9002(99)00730-5
Ice formation in model biological membranes is studied by SAXS and WAXS in the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as well-studied model biological membranes. A special cryostat is used for sample cooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in binary phospholipid/water and ternary phospholipid/cryoprotector/water systems in the condition of excess solvent. Ice formation in a binary phospholipid/water system creates an abrupt decrease of the membrane repeat distance by delta-d, so-called ice-induced dehydration of intermembrane space. The value of delta-d decreases as the cryoprotector concentration increases. The formation of ice does not influence the membrane structure (delta-d = 0) for cryoprotector mole fractions higher than 0.05.
Kiselev M. A.
Kisselev A. M.
Lesieur P.
Ollivon M.
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