Holographic Digital Fourier Microscopy for Selective Imaging of Biological Tissue

Physics – Optics

Scientific paper

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24 pages, 5 figures

Scientific paper

This paper presents an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high-resolution digital recording, digital Fourier microscopy (DFM) permits crucial flexibility in applying filtering to highlight scatterers of interest in the tissue. The high-resolution digital hologram is a result of the collation of Fourier holographic frames to form a large-size composite hologram. It is expected that DFM has an improved signal-to-noise ratio as compared to conventional direct digital imaging, e.g. phase microscopy, as applied to imaging of small-size objects. The demonstration of the Fourier filtering capacity of DFM using a biological phantom represents the main focus of this paper.

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