Gold/Silica biochips: applications to Surface Plasmon Resonance and fluorescence quenching

Details Gold/Silica biochips: applications to Surface Plasmon Resonance and fluorescence quenching Gold/Silica biochips: applications to Surface Plasmon Resonance and fluorescence quenching

2010-01-15

arxiv.org/abs/1001.2692v1

Laser Physics 19, 2 (2008) 252-58

Physics

Optics

Scientific paper

10.1134/S1054660X09020170

We report Gold/Silica biochips for low cost biosensor devices. Firstly, the study of biochemical interactions on silica by means of Surface Plasmon Resonance (SPR) is presented. Secondly, Gold/Silica biochips are employed to reduce the strong quenching that occurs when a fluorophore is close to the gold surface. Furthermore, the control of the Silica-like thickness allows optimizing the distance between the metallic surface and the fluorophore in order to enhance the fluorescent signal. These results represent the first steps towards highly sensitive, specific and low cost biosensors based, for example, on Surface Plasmon Coupled Emission (SPCE) techniques.

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