Getting DNA twist rigidity from single molecule experiments

Details Getting DNA twist rigidity from single molecule experiments Getting DNA twist rigidity from single molecule experiments

2004-02-17

arxiv.org/abs/q-bio/0402036v1

Biology

Quantitative Biology

Biomolecules

Scientific paper

We use an elastic rod model with contact to study the extension versus rotation diagrams of single supercoiled DNA molecules. We reproduce quantitatively the supercoiling response of overtwisted DNA and, using experimental data, we get an estimation of the effective supercoiling radius and of the twist rigidity of B-DNA. We find that unlike the bending rigidity, the twist rigidity of DNA seems to vary widely with the nature and concentration of the salt buffer in which it is immerged.

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