Genetic Control of β-Hydroxybutyric Dehydrogenase in Paramecium aurelia

Physics

Scientific paper

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Scientific paper

MAMMALIAN β-hydroxybutyric dehydrogenase is known to be firmly bound to the outer membrane of mitochondria and can be extracted by the neutral detergent `Triton X-100'1. In the investigation reported here the same enzyme was shown-by a similar extraction procedure-to be present in the mitochondria of the protozoan Paramecium aurelia. Extracts were made from a number of clones isolated from a natural population of P. aurelia (syngen 9), collected near Edinburgh and examined by starch gel electrophoresis. Two electrophoretically distinct forms of the enzyme were found in different clones and have been designated HBD-1 and HBD-2 respectively (Fig. 1a).

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