Physics – Biological Physics
Scientific paper
2005-03-11
Physics
Biological Physics
17 pages, 5 figures
Scientific paper
10.1529/biophysj.105.070128
Gel electrophoresis allows to separate knotted DNA (nicked circular) of equal length according to the knot type. At low electric fields, complex knots being more compact, drift faster than simpler knots. Recent experiments have shown that the drift velocity dependence on the knot type is inverted when changing from low to high electric fields. We present a computer simulation on a lattice of a closed, knotted, charged DNA chain drifting in an external electric field in a topologically restricted medium. Using a simple Monte Carlo algorithm, the dependence of the electrophoretic migration of the DNA molecules on the type of knot and on the electric field intensity was investigated. The results are in qualitative agreement with electrophoretic experiments done under conditions of low and high electric fields: especially the inversion of the behavior from low to high electric field could be reproduced. The knot topology imposes on the problem the constrain of self-avoidance, which is the final cause of the observed behavior in strong electric field.
Dietler Giovanni
Fleurant M.
Los Rios Paolo de
Stasiak Andrzej
Weber Carsten
No associations
LandOfFree
Gel Electrophoresis of DNA Knots in Weak and Strong Electric Fields does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Gel Electrophoresis of DNA Knots in Weak and Strong Electric Fields, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gel Electrophoresis of DNA Knots in Weak and Strong Electric Fields will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-345607