Biology
Scientific paper
Dec 2006
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2006oleb...36..605l&link_type=abstract
Origins of Life and Evolution of Biospheres, Volume 36, Issue 5-6, pp. 605-616
Biology
Cell-Free Protein Expression, Contingency, Folding, Liposomes, Minimal Cell, Minimal Genome, Never Born Proteins, Phage Display, Self-Reproduction
Scientific paper
The Never Born Proteins (NBPs) and the Minimal Cell projects are two currently developed research lines belonging to the field of synthetic biology. The first deals with the investigation of structural and functional properties of de novo proteins with random sequences, selected and isolated using phage display methods. The minimal cell is the simplest cellular construct which displays living properties, such as self-maintenance, self-reproduction and evolvability. The semi-synthetic approach to minimal cells involves the use of extant genes and proteins in order to build a supramolecular construct based on lipid vesicles. Results and outlooks on these two research lines are shortly discussed, mainly focusing on their relevance to the origin of life studies.
Chiarabelli Cristiano
Luisi Pier Luigi
Stano Pasquale
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