Physics – Chemical Physics
Scientific paper
2011-08-22
Atti Della "Fondazione Giorgio Ronchi" Anno LXVI, 2011 - N. 2
Physics
Chemical Physics
Scientific paper
We have measured the fluorescence spectrum for fluorescein solution in ethanol with concentration 1 {\times} 10-3 mol/liter at different temperatures from room temperature to freezing point of solvent, (T = 153, 183, 223, 253, and 303 K) using liquid nitrogen. Table curve 2D version 5.01 program has been used to determine the fitting curve and fitting equation for each fluorescence spectrum. Fourier series (3 {\times} 2) was the most suitable fitting equation for all spectra. Theoretical fluorescence spectrum of fluorescein in ethanol at T = 183K was calculated and compared with experimental fluorescence spectrum at the same temperature. There is a good similarity between them.
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