Fast fluorescence dynamics in non-ratiometric calcium indicators

Details Fast fluorescence dynamics in non-ratiometric calcium indicators Fast fluorescence dynamics in non-ratiometric calcium indicators

2008-07-17

arxiv.org/abs/0807.2756v2

Opt. Lett. 34 (2009) 362-364

Physics

Biological Physics

3 pages, 2 figures, figures revised, added chi-square goodness of fit

Scientific paper

10.1364/OL.34.000362

A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.

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