Expression of the Escherichia coli Cell Division Gene sep Cloned in a λ Charon Phage

Physics

Scientific paper

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Scientific paper

The Escherichia coli cell division gene sep, which probably codes for
one of the penicillin-binding proteins, has been cloned into λ
Charon 10 to form a viable sep+ transducing phage. After
injection with this hybrid phage, penicillin-binding protein 3 was
overproduced and incorporated into the E. coli inner membrane.

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