Evidence of Conformational Changes in Adsorbed Lysozyme Molecule on Silver Colloids

Details Evidence of Conformational Changes in Adsorbed Lysozyme Molecule on Silver Colloids Evidence of Conformational Changes in Adsorbed Lysozyme Molecule on Silver Colloids

2010-01-02

arxiv.org/abs/1001.0284v2

Physics

Biological Physics

15 pages, 6 figures

Scientific paper

In this article, we discuss metal-protein interactions in the Ag-lysozyme complex by spectroscopic measurements. The analysis of the variation in relative intensities of SERS bands reveal the orientation and the change in conformation of the protein molecules on the Ag surface with time. The interaction kinetics of metal-protein complexes has been analyzed over a period of three hours via both Raman and absorption measurements. Our analysis indicates that the Ag nanoparticles most likely interact with Trp-123 which is in close proximity to Phe-34 of the lysozyme molecule.

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