Entropic stabilization of proteins and its proteomic consequences

Details Entropic stabilization of proteins and its proteomic consequences Entropic stabilization of proteins and its proteomic consequences

2005-06-22

arxiv.org/abs/q-bio/0506032v1

Biology

Quantitative Biology

Biomolecules

Scientific paper

We report here a new entropic mechanism of protein thermostability due to residual dynamics of rotamer isomerization in native state. All-atom simulations show that Lysines have much greater number of accessible rotamers than Arginines in folded states of proteins. This finding suggests that Lysines would preferentially entropically stabilize the native state. Indeed we show in computational experiments that Arginine-to-Lysine amino acid substitutions result in noticeable stabilization of proteins. We then hypothesize that if evolution uses this physical mechanisms in its strategies of thermophilic adaptation then hyperthermostable organisms would have much greater content of Lysines in their proteomes than of comparable in size and similarly charged Arginines.. Consistent with that, high-throughput comparative analysis of complete proteomes shows extremely strong bias towards Arginine-to-Lysine replacement in hyperthermophilic organisms and overall much greater content of Lysines than Arginines in hyperthermophiles. This finding cannot be explained by GC compositional biases. Our study provides an example of how analysis of a delicate physical mechanism of thermostability helps to resolve a puzzle in comparative genomics as to why aminoacid compositions of hyperthermophilic proteomes are significantly biased towards Lysines but not Arginines

Affiliated with

Also associated with

No associations

Rating

Entropic stabilization of proteins and its proteomic consequences does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Entropic stabilization of proteins and its proteomic consequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Entropic stabilization of proteins and its proteomic consequences will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-480890