Physics – Biological Physics
Scientific paper
2011-02-03
Physics
Biological Physics
Accepted for publication in BBA - Biomembranes
Scientific paper
Electroporation is a physical method to induce the uptake of therapeutic drugs and DNA, by eukaryotic cells and tissues. The phenomena behind electro-mediated membrane permeabilization to plasmid DNA have been shown to be significantly more complex than those for small molecules. Small molecules cross the permeabilized membrane by diffusion whereas plasmid DNA first interacts with the electropermeabilized part of the cell surface, forming localized aggregates. The dynamics of this process is still poorly understood because direct observations have been limited to scales of the order of seconds. Here, cells are electropermeabilized in the presence of plasmid DNA and monitored with a temporal resolution of 2 ms. This allows us to show that during the first pulse application, plasmid complexes, or aggregates, start to form at distinct sites on the cell membrane. FRAP measurements show that the positions of these sites are remarkably immobile during the application of further pluses. A theoretical model is proposed to explain the appearance of distinct interaction sites, the quantitative increase in DNA and also their immobility leading to a tentative explanation for the success of electro-mediated gene delivery.
Dean David S.
Escoffre Jean-Michel
Favard Cyril
Portet Thomas
Rols Marie-Pierre
No associations
LandOfFree
Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-427358