Effective affinities in microarray data

Details Effective affinities in microarray data Effective affinities in microarray data

2006-05-04

arxiv.org/abs/q-bio/0605007v1

J. Phys.: Condens. Matter 18, S525(2006)

Biology

Quantitative Biology

Biomolecules

8 pages, 6 figures

Scientific paper

10.1088/0953-8984/18/18/S03

In the past couple of years several studies have shown that hybridization in Affymetrix DNA microarrays can be rather well understood on the basis of simple models of physical chemistry. In the majority of the cases a Langmuir isotherm was used to fit experimental data. Although there is a general consensus about this approach, some discrepancies between different studies are evident. For instance, some authors have fitted the hybridization affinities from the microarray fluorescent intensities, while others used affinities obtained from melting experiments in solution. The former approach yields fitted affinities that at first sight are only partially consistent with solution values. In this paper we show that this discrepancy exists only superficially: a sufficiently complete model provides effective affinities which are fully consistent with those fitted to experimental data. This link provides new insight on the relevant processes underlying the functioning of DNA microarrays.

Affiliated with

Also associated with

No associations

Rating

Effective affinities in microarray data does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Effective affinities in microarray data, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Effective affinities in microarray data will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-300092