DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments

Details DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments

2007-12-18

arxiv.org/abs/0712.2919v1

The European Physical Journal E 24 (2007) 9-18

Physics

Biological Physics

Scientific paper

10.1140/epje/i2007-10205-5

We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda) using the smotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.

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