Development of plant protoplasts during the IML-1 mission

Details Development of plant protoplasts during the IML-1 mission Development of plant protoplasts during the IML-1 mission

Aug 1994

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1994adspr..14..189r&link_type=abstract

Advances in Space Research, Volume 14, Issue 8, p. 189-196.

Physics

1

Scientific paper

During the 8 day IML-1 mission, regeneration of cell walls and cell divisions in rapeseed protoplasts were studied using the Biorack microscope onboard the Space Shuttle ``Discovery''. Samples from μ-g and 1g protoplast cultures were loaded on microscope slides. Visual microscopic observations were reported by the payload specialist Roberta Bondar, by down-link video transmission and by use of a microscope camera. Protoplasts grown under μ-g conditions do regenerate cell walls but to a lesser extent than under 1 g. Cell divisions are delayed under μ-g. Few cell aggregates with maximum 4-6 cells per aggregate are formed under μ-g conditions, indicating that microgravity may have a profound influence on plant cell differentiation.

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