Physics
Scientific paper
Jun 1998
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1998rapc...52..141k&link_type=abstract
Radiation Physics and Chemistry, Volume 52, Issue 1-6, p. 141-144.
Physics
Scientific paper
Microgel electrophoresis of single cells (DNA comet assay) has been investigated to detect irradiation treatment of some food samples. These samples of fresh and frozen rainbow trout, red lentil, gram and sliced almonds were irradiated to 1 or 2 kGy using 10 MeV electron beam from a linear accelerator. Rainbow trout samples yielded good results with samples irradiated to 1 or 2 kGy showing fragmentation of DNA and, therefore, longer comets with no intact cells. Unirradiated samples showed shorter comets with a significant number of intact cells. For rainbow trout stored in a freezer for 11 days the irradiated samples can still be discerned by electrophoresis from unirradiated samples, however, the unirradiated trouts also showed some longer comets besides some intact cells. Radiation treatment of red lentils can also be detected by this method, i.e. no intact cells in 1 or 2 kGy irradiated samples and shorter comets and some intact cells in unirradiated samples. However, the results for gram and sliced almond samples were not satisfactory since some intact DNA cells were observed in irradiated samples as well. Probably, incomplete lysis has led to these deviating results.
Delincée Henry
Khan Hasan M.
No associations
LandOfFree
Detection of Irradiation Treatment of Foods Using DNA `Comet Assay' does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Detection of Irradiation Treatment of Foods Using DNA `Comet Assay', we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Detection of Irradiation Treatment of Foods Using DNA `Comet Assay' will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1447685