Delivery of optical contrast agents using Triton-X100, part 1: reversible permeabilization of live cells for intracellular labeling

Details Delivery of optical contrast agents using Triton-X100, part 1: reversible permeabilization of live cells for intracellular labeling Delivery of optical contrast agents using Triton-X100, part 1: reversible permeabilization of live cells for intracellular labeling

Mar 2009

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2009jbo....14b1012v&link_type=abstract

Journal of Journal of Biomedical Optics, Volume 14, Issue 2, pp. 021012-021012-10 (2009).

Physics

1

Biomedical Materials, Cellular Biophysics, Permeability, Proteins

Scientific paper

Effective delivery of optical contrast agents into live cells remains a significant challenge. We sought to determine whether Triton-X100, a detergent commonly used for membrane isolation and protein purification, could be used to effectively and reversibly permeabilize live cells for delivery of targeted optical contrast agents. Although Triton-X100 is widely recognized as a good cell permeabilization agent, no systematic study has evaluated the efficiency, reproducibility, and reversibility of Triton-X100-mediated permeabilization in live mammalian cells. We report a series of studies to characterize macromolecule delivery in cells following Triton-X100 treatment. Using this approach, we demonstrate that molecules ranging from 1 to 150 kDa in molecular weight can be reproducibly delivered into live cells by controlling the moles of Triton-X100 relative to the number of cells to be treated. When Triton-X100 is administered at or near the minimum effective concentration, cell permeabilization is generally reversed within 24 h, and treated cells continue to proliferate and show metabolic activity during the restoration of membrane integrity. We conclude that Triton-X100 is a promising permeabilization agent for efficient and reproducible delivery of optical contrast agents into live mammalian cells.

Affiliated with

Also associated with

No associations

Rating

Delivery of optical contrast agents using Triton-X100, part 1: reversible permeabilization of live cells for intracellular labeling does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Delivery of optical contrast agents using Triton-X100, part 1: reversible permeabilization of live cells for intracellular labeling, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Delivery of optical contrast agents using Triton-X100, part 1: reversible permeabilization of live cells for intracellular labeling will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-1264276