Continuous monitoring of membrane protein micro-domain association during cell signaling

Details Continuous monitoring of membrane protein micro-domain association during cell signaling Continuous monitoring of membrane protein micro-domain association during cell signaling

2011-01-26

arxiv.org/abs/1101.5087v1

Physics

Biological Physics

13 pages, 5 figures, currently being reviewed by journal

Scientific paper

Central to understanding membrane bound cell signaling is to quantify how the membrane ultra-structure consisting of transient spatial domains modulates signaling and how the signaling influences this ultra-structure. Yet, measuring the association of membrane proteins with domains in living, intact cells poses considerable challenges. Here, we describe a non-destructive method to quantify protein-lipid domain and protein cytoskeleton interactions in single, intact cells enabling continuous monitoring of the protein domains interaction over time during signaling.

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