Construction of Chimeric Phages and Plasmids Containing the Origin of Replication of Bacteriophage Lambda

Physics

Scientific paper

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Scientific paper

Segments of the replication control region of bacteriophage lambda (λ ) and λ mutants defective in replication were attached in vitro to the φ 80 phage vector Charon 3 and to the plasmid vector mini Col El (pVH51). The chimeric phages and plasmids have been used to localize the origin of λ DNA replication and to facilitate a structural analysis of the λ replicator.

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