Common Arc Method for Diffraction Pattern Orientation

Details Common Arc Method for Diffraction Pattern Orientation Common Arc Method for Diffraction Pattern Orientation

2011-05-10

arxiv.org/abs/1105.1926v1

Physics

Data Analysis, Statistics and Probability

16 pages, 10 figures

Scientific paper

Very short pulses of x-ray free-electron lasers opened the way to obtain diffraction signal from single particles beyond the radiation dose limit. For 3D structure reconstruction many patterns are recorded in the object's unknown orientation. We describe a method for orientation of continuous diffraction patterns of non-periodic objects, utilizing intensity correlations in the curved intersections of the corresponding Ewald spheres, hence named Common Arc orientation. Present implementation of the algorithm optionally takes into account the Friedel law, handles missing data and is capable to determine the point group of symmetric objects. Its performance is demonstrated on simulated diffraction datasets and verification of the results indicates high orientation accuracy even at low signal levels. The Common Arc method fills a gap in the wide palette of the orientation methods.

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