Cloning Human Fetal γ Globin and Mouse α -Type Globin DNA: Preparation and Screening of Shotgun Collections

Details Cloning Human Fetal γ Globin and Mouse α -Type Globin DNA: Preparation and Screening of Shotgun Collections Cloning Human Fetal γ Globin and Mouse α -Type Globin DNA: Preparation and Screening of Shotgun Collections

Dec 1978

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1978sci...202.1279b&link_type=abstract

Science, Volume 202, Issue 4374, pp. 1279-1284

Physics

17

Scientific paper

Shotgun collections of Charon 3A bacteriophages containing Eco RI fragments of human and mouse DNA were constructed with the use of in vitro packaging. Plaques were screened by hybridization, and globin-specific clones were isolated from both human (Charon 3AHs51.1) and mouse (Charon 3AMm30.5). The fragments cloned were detected in unfractionated genomic DNA by the Southern method of hybridization.

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