Characterizing and tracking single colloidal particles with video holographic microscopy

Details Characterizing and tracking single colloidal particles with video holographic microscopy Characterizing and tracking single colloidal particles with video holographic microscopy

2007-12-11

arxiv.org/abs/0712.1738v1

Physics

Optics

6 pages, 3 figures

Scientific paper

10.1364/OE.15.018275

We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and refractive index to within 1%, and simultaneously to measure its three-dimensional position with nanometer in-plane precision and 10 nanometer axial resolution.

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