Carbohydrates from Santa Barbara Basin sediments: Gas chromatographic-mass spectrometric analysis of trimethylsilyl derivatives

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Two Santa Barbara Basin marine sediments were hydrolyzed and the concentration and identification of their constituent carbohydrate moieties were determined. The ages of these sediments were estimated to be ~50 yr and ~750 yr, respectively, by counting seasonal varves by X-ray techniques. Thin-layer chromatograms developed in ethyl acetate, pyridine and water or in n-propanol, ethyl acetate and water indicated the major constituents of the desalted hydrolysates. Combined capillary gas chromatography-mass spectrometry of trimethylsilyl (TMS) derivatives of the hydrolysates resulted in the identification of glucose, galactose, mannose, ribose, xylose, arabinose, fucose and rhamnose, as well as mannitol and the TMS derivatives of stearic and palmitic acids. The two marine sediments contained the same carbohydrate moieties with a lower total concentration in the ~750-yr sample; some of the sugars seem to be similar to those commonly found in marine algae. The total organic content of the two sediments was approximately the same.

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