Breakdown of thermodynamic equilibrium for DNA hybridization in microarrays

Details Breakdown of thermodynamic equilibrium for DNA hybridization in microarrays Breakdown of thermodynamic equilibrium for DNA hybridization in microarrays

2010-01-14

arxiv.org/abs/1001.2428v1

Phys. Rev. E 81, 012901 (2010)

Biology

Quantitative Biology

Biomolecules

4 pages, 4 figures

Scientific paper

10.1103/PhysRevE.81.012901

Test experiments of hybridization in DNA microarrays show systematic deviations from the equilibrium isotherms. We argue that these deviations are due to the presence of a partially hybridized long-lived state, which we include in a kinetic model. Experiments confirm the model predictions for the intensity vs. free energy behavior. The existence of slow relaxation phenomena has important consequences for the specificity of microarrays as devices for the detection of a target sequence from a complex mixture of nucleic acids.

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