Biology – Quantitative Biology – Biomolecules
Scientific paper
2005-01-06
Biology
Quantitative Biology
Biomolecules
32 pages, 12 figures, 3 tables
Scientific paper
The lack of specificity in microarray experiments due to non-specific hybridization raises a serious problem for the analysis of microarray data because the residual chemical background intensity is not related to the expression degree of the gene of interest. We analyzed the concentration dependence of the signal intensity of perfect match (PM) and mismatch (MM) probes in terms using a microscopic binding model using a combination of mean hybridization isotherms and single base related affinity terms. The signal intensities of the PM and MM probes and their difference are assessed with regard to their sensitivity, specificity and resolution for gene expression measures. The presented theory implies the refinement of existing algorithms of probe level analysis to correct microarray data for non-specific background intensities.
Binder Hans
Kirsten Toralf
Preibisch Stephan
No associations
LandOfFree
Base pair interactions and hybridization isotherms of matched and mismatched oligonucleotide probes on microarrays does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Base pair interactions and hybridization isotherms of matched and mismatched oligonucleotide probes on microarrays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Base pair interactions and hybridization isotherms of matched and mismatched oligonucleotide probes on microarrays will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-293201