Axial localization of luminophores by partial coherence interferometry

Details Axial localization of luminophores by partial coherence interferometry Axial localization of luminophores by partial coherence interferometry

2006-06-08

arxiv.org/abs/physics/0606080v1

Biophotonics Micro- and Nano-Imaging (2004) 51

Physics

Biological Physics

Scientific paper

We propose a solution for increasing the axial resolution of confocal microscopes. In the experimental set-up described in this paper an interference phenomenon between two counterpropagating beams is used to determine the axial position of a luminophore. The optical path difference between the two waves, which is related to the position of the luminophore, is recovered thanks to a second interferometer by using partial coherence interferometry demodulation technique. The proposed solution can find applications in biology for localizing with nanometric resolution a small number of tagged species.

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